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#5227 Apr 22 2011 at 7:58 AM Rating: Good
PigtailsOfDoom wrote:
Kalivha wrote:
The pictures I've seen from the Edinburgh Beltane indicate a lot of people naked with colourful body paint. <3


Yeah, that just makes me want to go more. =x


Actually, judging from your pictures on FetLife, you'd... you're like a PERFECT match with the Edinburgh/Beltane/FetSoc crowd. Heh.
#5228 Apr 22 2011 at 9:44 AM Rating: Good
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PigtailsOfDoom wrote:
Oh and Aeth, out of curiosity, do you still want the threesome when you're sober? :D
Sure. Smiley: sly

Edit: Also, you should add me on Facebook.



And today was awesome. We went to De Hoge Veluwe and basically spent the day cycling around in the forest.
Edit 2: I want to add the above quote to my sig but I probably shouldn't.

Edited, Apr 22nd 2011 7:47pm by Aethien
#5229 Apr 22 2011 at 1:25 PM Rating: Good
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Post more you cnuts. I'm bored.

Edited, Apr 22nd 2011 9:27pm by Aethien
#5230 Apr 22 2011 at 1:57 PM Rating: Good
Kalivha wrote:
PigtailsOfDoom wrote:
Kalivha wrote:
The pictures I've seen from the Edinburgh Beltane indicate a lot of people naked with colourful body paint. <3


Yeah, that just makes me want to go more. =x


Actually, judging from your pictures on FetLife, you'd... you're like a PERFECT match with the Edinburgh/Beltane/FetSoc crowd. Heh.


Heh, that's funny. One of my friends here actually lived in Scotland for a few months when she moved there to be with a guy she met online. She's got some really funny stories to tell about his family. He ended up being a cheating *** hole, but apparently there were some amusing karaoke events. One of his relatives thought that Americans never fought the English. :D

If you guys want me to add you on facebook, just send me your emails or something through pm.
#5231 Apr 22 2011 at 1:57 PM Rating: Good
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I've not really got much to post about, other than the fact that I just remembered the song I got punched in the face to. Good times.
#5232 Apr 22 2011 at 2:30 PM Rating: Excellent
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Sgriob wrote:
I've not really got much to post about


Me either. Smiley: frown

Unless you care about using summed MS/MS ion current intensities to estimate protein abundance in a complex background; but I'm guessing you don't. Smiley: tongue
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#5233 Apr 22 2011 at 2:33 PM Rating: Good
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someproteinguy wrote:
Unless you care about using summed MS/MS ion current intensities to estimate protein abundance in a complex background; but I'm guessing you don't. Smiley: tongue
Kali probably does.
And I wouldn't mind trying to understand it.
#5234 Apr 22 2011 at 2:34 PM Rating: Excellent
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Also I have Peeps. Smiley: nod
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#5235 Apr 22 2011 at 2:42 PM Rating: Excellent
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His Excellency Aethien wrote:
someproteinguy wrote:

Unless you care about using summed MS/MS ion current intensities to estimate protein abundance in a complex background; but I'm guessing you don't. Smiley: tongue

Kali probably does.
And I wouldn't mind trying to understand it.


Sweet.

Here's your introductory reading assignment. It covers the basics. You have to know what an MS/MS spectra is before you can discuss whether or not it generates useful quantitative information. Smiley: wink

Have you taken some university level biology/genetics stuff?

Edited, Apr 22nd 2011 1:44pm by someproteinguy
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#5236 Apr 22 2011 at 2:49 PM Rating: Good
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someproteinguy wrote:
Have you taken some university level biology/genetics stuff?
The last time I paid attention to biology was sex ed and that's 9 years ago Smiley: lol


That said, the first page is easy to read and I actually recognize a bunch of words from the big bang theory. Smiley: grin
Edit: not quite understanding it yet but I can follow it so I'll read the rest when I've had a good amount of sleep.

Edited, Apr 22nd 2011 10:53pm by Aethien
#5237 Apr 22 2011 at 2:54 PM Rating: Excellent
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Quote:
someproteinguy wrote:

Have you taken some university level biology/genetics stuff?

The last time I paid attention to biology was sex ed and that's 9 years ago Smiley: lol


That said, the first page is easy to read and I actually recognize a bunch of words from the big bang theory. Smiley: grin


Perfect.

I have hope now; you have more background in this then half the people I try to explain it to. Smiley: lol
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#5238 Apr 22 2011 at 3:02 PM Rating: Good
I worked in a stem cell lab a few years ago.

We didn't actually do any mass spectrometry there, but I've been to a few analytical chemistry conferences here in the UK (solid state MRS <3), and I've sat exams about this stuff (both the analytical part and the biology and chemistry viewpoints on proteins) in the last 2 academic years and in chemistry I'm good enough to need a C in my final to get an A in the end. ;)


Pigtails, I'll post you my FB link on Fetlife, heh. And done.
#5239 Apr 22 2011 at 3:07 PM Rating: Good
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See, I told you Kali would like it ^^
Also, who did you try to explain it to that I'm above average? I'm mostly useless for anything other than making pretty pictures. Smiley: lol

Edited, Apr 22nd 2011 11:08pm by Aethien
#5240 Apr 22 2011 at 3:21 PM Rating: Good
You don't realise how ******* stupid and uneducated the average person is, dear. Being European gives you, like, an instant advantage.
#5241 Apr 22 2011 at 3:26 PM Rating: Good
I love your facebook pic Kali, the mustache is hilarious! :D
#5242 Apr 22 2011 at 3:27 PM Rating: Excellent
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His Excellency Aethien wrote:
Also, who did you try to explain it to that I'm above average?


Friends, family, anyone who's ever tried to ask me "what do you do for a living?" in casual conversation. Smiley: rolleyes

Let's just say I've gone to saying "I do medical research" and left it at that. But even among relatively educated people it'd hard to find anyone who knows what Proteomics is though (firefox doesn't know either Smiley: wink) as it's still a pretty new field. The first computer software to do this kind of work was written around 1994, and the field didn't really receive it's 'name' until 2004 or so.
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#5243 Apr 22 2011 at 3:29 PM Rating: Good
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Kalivha wrote:
You don't realise how @#%^ing stupid and uneducated the average person is, dear. Being European gives you, like, an instant advantage.
I know that most people are retarded, but I figured that with Protein being a scientist and all I wouldn't end up above average.
PigtailsOfDoom wrote:
I love your facebook pic Kali, the mustache is hilarious! :D
It's horrible and it freaks me out a little when I see it. Smiley: frown
#5244 Apr 22 2011 at 3:30 PM Rating: Excellent
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Kalivha wrote:
You don't realise how @#%^ing stupid and uneducated the average person is, dear.


Smiley: lol

Okay, I wouldn't quite go that far, but sure. Smiley: grin

Really a lot of people from where I grew up either became farmers or loggers. There's some bright apples (and not so bright ones) in that bunch; but they don't have a shred of context to start a conversation from. Smiley: frown
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#5245 Apr 22 2011 at 3:32 PM Rating: Good
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someproteinguy wrote:
Kalivha wrote:
You don't realise how @#%^ing stupid and uneducated the average person is, dear.


Smiley: lol

Okay, I wouldn't quite go that far, but sure. Smiley: grin


I would agree with Kal's sentiment.
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idiggory wrote:
Drinking at home. But I could probably stand to get laid.
#5246 Apr 22 2011 at 3:36 PM Rating: Good
someproteinguy wrote:
Kalivha wrote:
You don't realise how @#%^ing stupid and uneducated the average person is, dear.


Smiley: lol

Okay, I wouldn't quite go that far, but sure. Smiley: grin

Really a lot of people from where I grew up either became farmers or loggers. There's some bright apples (and not so bright ones) in that bunch; but they don't have a shred of context to start a conversation from. Smiley: frown


The variation with my high school class is odd. There's a fair amount of people who went on to college and now have good careers and stuff, but there's also quite a few who are now stay at homes mom's or have part time jobs, or just are complete slackers who are doing nothing with their lives. I don't think anyone is a farmer or a logger, but then again the logging industry in the town I grew up in is pretty much dead.
#5247 Apr 22 2011 at 3:43 PM Rating: Excellent
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PigtailsOfDoom wrote:
I don't think anyone is a farmer or a logger, but then again the logging industry in the town I grew up in is pretty much dead.


Yeah, logging isn't what it used to be there either. I lived down the street from the only mill that was still open in town; it was still pretty busy though. There was also a gravel pit and a coal mine, plus the traffic from the interstate funding all kinds of fast food places and hotels. In the end nothing that screamed "educated workforce" really. Smiley: frown

The only 'chemists' in town were the ones that cooked their own meth. Smiley: rolleyes
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#5248 Apr 22 2011 at 3:44 PM Rating: Good
Protein, do you actually try creating new proteins, then? Because that'd be so cool.



As for the people from my past... hmm.
My best friend from primary school is in his first year of applied computer science at university; most people from my secondary school are finishing university by now as everything from vicars to mechanical engineers.
My best friend from that school is actually... she just dropped out of physiotherapy school. She's more problematic than I am, actually.
Most people at college that I actually talk to will go on to university after this year.

The few people I've sort of kept track of from my high school in the US married at 18 and have kids now, and no career to speak of. Heh.
#5249 Apr 22 2011 at 4:00 PM Rating: Excellent
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Kalivha wrote:
Protein, do you actually try creating new proteins, then? Because that'd be so cool.


Naw, not so much. Darn and I wanted to be cool too... Smiley: frown

Most of it is biomarker discovery and validation. It's fairly simple on the surface. You take something like healthy mouse brain and diseased mouse brain, grind them up, extract the proteins, and give them to us to run them into the instrument. We use a rather complex data analysis pipeline and do some statistics and stuff on what pops out. In the end you've spent something like $15,000 and we given you a list of biomarker candidates. If you did a good experiment you could spend the rest of your career testing antibodies, validating, and following up on the results. With a little luck you'll find something interesting and make millions. If not, well, you helped pay my bills at least. Smiley: grin

Edited, Apr 22nd 2011 3:12pm by someproteinguy
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#5250 Apr 22 2011 at 4:00 PM Rating: Excellent
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PigtailsOfDoom wrote:
One of his relatives thought that Americans never fought the English. :D

Compared to the Scots, we haven't.

Hell, the 1977 Home International at Wembley was probably rougher on England than the entire War of 1812...
#5251 Apr 22 2011 at 4:36 PM Rating: Good
Yeah there's not really much educated work force in the town I grew up either. All the people who have gone on to bigger and better things didn't come back to the town once they graduated. Unless they were coming back to teach, which a few have done.

It's true the Scots fought the English for hundreds of years, where as we've only fought them twice, but I still thought that was funny. Especially when she pointed out that we had fought them and won and he asked how we won. She replied "Well, they wore red coats in a forest." His response was "Damn it! I knew we missed our chance!"

It's a lot funnier to hear her tell the story. She's a great story teller. She's actually started doing stand up at a local drag show once a month.
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